Strains from Pathway Biologic’s collection are useful in a variety of applications, including but not limited to biotechnology, agricultural crop production, and plant growth.
Pathway’s Microbial Diversity
Pathway’s microbial collection consists of beneficial microbial strains and beneficial fungi. The most important beneficial bacteria belong to the genus Bacillus and related taxa. Among the genus Bacillus we have over 30 different species and within each species, we have selected and cultivated many different strains. In addition, the diversity of Pathway’s bacterial collection is enhanced by strains of Actynomycetes and Pseudomonas. Among the beneficial fungi in our collection, Trichoderma spp., Paecilomyces lilacinus and Beauveria bassianna dominate.
Pathway’s Molecular Approach
Pathway’s beneficial bacteria are identified using the analysis of nucleotide sequences of the 16s ribosomal DNA gene (16s rDNA). The use of 16S rDNA gene sequences to study bacterial phylogeny and taxonomy is the best-characterized and most consistent genetic marker used, due to its universal presence and conserved nature.
Pathway’s procedure consists of DNA extraction from unknown strains, followed by PCR amplification, using time-tested primers, and visualization of the 16s rDNA region. Finally, the sequence for the 16s rDNA region is edited and compared to sequences available in the Ribosomal Database Project. Pathway Beneficial fungi have been identified using morphological features and sequencing of the internal transcribed space (ITS) regions.
To achieve the most consistent strains possible, we use rep-PCR for molecular fingerprinting of Pathway’s strains. Over 100 rep-PCR patterns have been generated and saved in our library. We compare clustering generated from data during the microbial spore production process with the clustering saved in our library. This comparison allows us to ensure reproducibility, purity, and presence of the right strain all along the production process.
Multilocus sequence typing (MLST) is being utilized by Pathway’s researchers to identify and characterize members of the Bacillus cereus group (B.c.). The B.c. group is composed of closely related species that are described as pathogenic organisms as well as bacteria with industrial applications (B. thuringiensis and B. mycoides). MLST is based on the sequence of a number of housekeeping genes spread around the bacterial chromosome. This standard method allows characterizing and identifying B c. group species at the molecular level.
Pathway’s Strain Bioactivity
Pathway’s inoculants are made with beneficial microorganisms that were originally isolated from the soil environment. Each beneficial microorganism is characterized for metabolite (primary and secondary) production and expression of plant growth promoting traits. An important ecological aspect of beneficial microbes is their ability to colonize and multiply on plant roots. Once Pathway’s inoculants are applied to the soil, the microbial strains will reproduce on plant roots and affect their beneficial traits. These beneficial effects are plant growth promotion, availability of nutrients for plant uptake, and production of biosurfactants, among others.
Pathway’s characterization goes beyond lab evaluations, our strain are tested under greenhouse and field conditions, always looking for the best growth promotion and nutrient cycling.
Pathway’s laboratory is working with Plant Growth Promoting Rhizobacteria for future of Bio-Control Products
In 1978 the term plant growth-promoting rhizobacteria (PGPR) was introduced by Kloepper and Schroth (1978) to refer to those beneficial bacteria that aggressively colonize plant roots (Kloepper, 1993; Schroth and Becker, 1990). Benefits produced by PGPR have been widely documented on different plant species including crops, ornamentals, vegetables, and forestry (Zahir et al., 2004).
Mechanisms by which PGPR produce their beneficial effects have been classified in two different groups: indirect and direct mechanisms. Indirect mechanisms relate to growth promotion as a result of providing a more active soil microbiome and healthier soil environment. Direct mechanisms are those in which rhizobacteria promote growth by producing plant growth compounds (phytohormones) or solubilizing minerals (nitrogen, phosphorous) (Cattelan et al., 1999). Indirect mechanisms include a. antibiotic production: antibiotics are defined as organic, low molecular weight compounds produced by microorganisms that are deleterious to the growth or metabolic activities of others (Raaijmakers et al., 2002). b. Siderophore production: siderophores are a low molecular iron transport agent that naturally chelates iron improving.
Read Next: Pathway’s Inoculants